Products

LTC

LTC is an interactive package for physical mapping of fingerprinted BAC libraries.

LTC can be used for construction, verification, elongation, merging, and “curing” of contigs and MTPs.

Features:

• More effective detection of problematic clones and clone overlaps in comparison to standard FPC software (based on exploring of parallel clone overlaps)
• Possibility to detect false contigs by checking the topological structure of clone overlaps
• Construction of reliable and longer contigs compared to FPC
• Reviewing, editing, repairing and elongation of contigs constructed with FPC
• MTP selection with taking into account clone overlaps (hence reducing the risk of gaps)
• Possibility to automatically reselect MTP with maximal using of already selected MTP clones with especial preference for some clones (e.g., already sequenced)
• Ordering of long sequence contigs of individual clones or pools of overlapping clones
• Mapping of long sequence contigs (>15kbp) to clones based on in silico fingerprinting
• Mapping of fingerprinted BACs/physical maps to long and very long sequence contigs
• Interactive editing of contigs and MTP facilitated by graphical visualization
• User-friendly interface for contig elongation and merging
• More powerful detection of putatively problematic clones
• Tools for repairing of gaps observed in the MTP
• Possibility to work with large BAC libraries (e.g., 106 clones)
• Taking into account different versions of fingerprinting
• Taking into account clone sequence for contig and MTP editing
• Tools assisting in the identification of positive clones from the set of positive 3D-pools